IGEM:metu/2009/Notebook/wound dressing/2009/10/18
 WOUND DRESSING
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
18.10.2009(Group 2) 1.Directly testing the oxygen promoter in the presence or absence of oxygen.We have prepared 4 set of tubes in the presence or absence of oxygen and we compared the growth of bacterias by using spectrophotometer. OR, testing the hemoglobin activity; we thought that if the gene coding for hemoglobin is working it means that our oxygen promoter is also working. (Group 1) 2. Centrifuge of supernatant + ammonium sulfate, 14000 rpm for 30 minutes. 3. Preparation of potassium phosphate buffer ( pH 6.6) 4. Resuspend the pellet in potassium phosphate buffer, 5ml,0.1 M and pH 6.6 5.Dialyzing them (Lard, Lard + ABC and Only top 10) in a nitrocellulose tube at 4 centigrade degrees in 500 microliter potassium phosphate buffer stirring for overnight. |
|
