Objective
- The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units.
Solutions
For E. coli Transformation
LB medium
|
|
Yeast Extract |
5 g/L
|
NaCl |
5 g/L
|
Casein Peptone (pH 7.2) |
10 g/L
|
Distilled water |
1 L
|
SOC medium
|
|
Tryptone |
20 g/L
|
Yeast Extract |
5 g/L
|
MgSO4 |
4.8 g/L
|
Dextrose |
3.603 g/L
|
NaCl |
0.5 g/L
|
KCl |
0.186 g/L
|
Distilled water |
|
Materials
|