IGEM:iGEM gto mx/2009/Notebook/Interlab 2016/Entry Base: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
==Objective==
===Objective===
*The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units.
*The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units.


===Materials ===
=== For E. coli Transformation ===
{|{{table}}
| align="center" style="background:#f0f0f0;"|''' SOC medium'''
| align="center" style="background:#f0f0f0;"|'''Amount'''
|-
| Tryptone || 20 g/L
|-
| Yeast Extract || 5 g/L
|-
|-
| MgSO4 || 4.8 g/L
|-
| Dextrose || 3.603 g/L
|-
|-
| NaCl || 0.5 g/L
|-
|-
| KCl || 0.186 g/L
|-
|-
| Distilled water ||
|-
|}




Revision as of 12:05, 22 August 2016

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Objective

  • The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units.

Materials

For E. coli Transformation

SOC medium Amount
Tryptone 20 g/L
Yeast Extract 5 g/L
MgSO4 4.8 g/L
Dextrose 3.603 g/L
NaCl 0.5 g/L
KCl 0.186 g/L
Distilled water



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:iGEM_gto_mx/2009/Notebook/Interlab_2016/Entry_Base&oldid=946296"