IGEM:Virginia 2012/Protocols/SSM Media Preparation
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| + | ==Materials== | ||
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| CaCl<sub>2</sub> 2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg | | CaCl<sub>2</sub> 2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg | ||
|} | |} | ||
| + | |||
| + | ==Procedure== | ||
| + | * Add 2.9g of NaCl to a 1 L bottle. | ||
| + | * Add in 25 mL of 7.5 pH Tris-Cl | ||
| + | * Bring this to a volume of 500 mL by adding distilled water. | ||
| + | * Autoclave (large liquid). | ||
| + | |||
| + | ==Notes== | ||
| + | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
| + | |||
| + | <!-- Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.--> | ||
| + | |||
| + | ==References== | ||
| + | <!-- If this protocol has papers or books associated with it, list those references here.--> | ||
| + | |||
| + | ==Contact== | ||
| + | *Who has experience with this protocol? | ||
| + | |||
| + | or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | ||
| + | |||
| + | <!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --> | ||
| + | [[Category:Protocol]] | ||
| + | <!-- Move the relevant categories above this line to tag your protocol with the label | ||
| + | [[Category:Protocol]] | ||
| + | |||
| + | [[Category:Needs attention]] | ||
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| + | [[Category:In vitro]] | ||
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| + | [[Category:In vivo]] | ||
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| + | [[Category:In silico]] | ||
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| + | [[Category:DNA]] | ||
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| + | [[Category:RNA]] | ||
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| + | [[Category:Protein]] | ||
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| + | [[Category:Chemical]] | ||
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| + | [[Category:Escherichia coli]] | ||
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| + | [[Category:Yeast]] | ||
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Revision as of 14:24, 31 May 2012
Contents |
Materials
| BASAL MEDIUM to make | 1 Liter | 2 Liters | 4 Liters | 5 Liters |
| Glutamic acid, monosodium salt, 1H2O | 10.72g | 21.44g | 42.88g | 53.60g |
| Trisma Base | 1.525g | 3.05g | 6.10g | 7.63g |
| NaCl | 2.50g | 5.00g | 10.00g | 12.50g |
| KH2PO4 | 0.50g | 1.00g | 2.00g | 2.50g |
| KCl | 0.20g | 0.40g | 0.80g | 1.00g |
| MgCl2 6H2O | 100mg | 200mg | 400mg | 500mg |
| CaCl2 2H2O | 20mg | 40mg | 80mg | 100mg |
Procedure
- Add 2.9g of NaCl to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
