http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Fri, 24 May 2013 20:01:28 GMT http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&diff=615619&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:40, 26 July 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Add <del class="diffchange diffchange-inline">2.9g of </del>NaCl to a 1 L bottle.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Add <ins class="diffchange diffchange-inline">the </ins>NaCl <ins class="diffchange diffchange-inline">and MgSO4 </ins>to a 1 L bottle.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*&nbsp; Add in 25 mL of 7.5 pH Tris-Cl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*&nbsp; Add in 25 mL of 7.5 pH Tris-Cl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Bring this to a volume of 500 mL by adding distilled water.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Bring this to a volume of 500 mL by adding distilled water.</div></td></tr> <!-- diff generator: internal 2013-05-24 20:01:29 --> </table> Thu, 26 Jul 2012 13:40:57 GMT Joseph J. Muldoon http://openwetware.org/wiki/Talk:IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&diff=604819&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:09, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 10:</td> <td colspan="2" class="diff-lineno">Line 10:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*&nbsp; Add in 25 mL of 7.5 pH Tris-Cl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*&nbsp; Add in 25 mL of 7.5 pH Tris-Cl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Bring this to a volume of 500 mL by adding distilled water.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Bring this to a volume of 500 mL by adding distilled water.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Be happy</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Autoclave (large liquid)</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr> <!-- diff generator: internal 2013-05-24 20:01:29 --> </table> Wed, 30 May 2012 18:09:18 GMT Jacqueline K. Grimm http://openwetware.org/wiki/Talk:IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&diff=604813&oldid=prev <p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:46, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Add 2.9g of NaCl to a 1 L bottle.</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*&nbsp; Add in 25 mL of 7.5 pH Tris-Cl</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Bring this to a volume of 500 mL by adding distilled water.</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Be happy.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr> <!-- diff generator: internal 2013-05-24 20:01:29 --> </table> Wed, 30 May 2012 17:46:34 GMT Alex Zorychta http://openwetware.org/wiki/Talk:IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&diff=604812&oldid=prev <p><span class="autocomment">Materials</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:45, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*2.9 g NaCl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*2.9 g NaCl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*0.49 g MgSO4</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*0.49 g MgSO4</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*25 mL 1 M Tris*Cl pH 7.5</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*25 mL 1 M Tris*Cl <ins class="diffchange diffchange-inline">at </ins>pH 7.5</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">BTV </del>500 mL <del class="diffchange diffchange-inline">dH2O</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">less than </ins>500 mL <ins class="diffchange diffchange-inline">of distilled H2O</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td></tr> <!-- diff generator: internal 2013-05-24 20:01:29 --> </table> Wed, 30 May 2012 17:45:10 GMT Alex Zorychta http://openwetware.org/wiki/Talk:IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation&diff=604810&oldid=prev <p>New page: ==Materials== *2.9 g NaCl *0.49 g MgSO4 *25 mL 1 M Tris*Cl pH 7.5 *BTV 500 mL dH2O ==Procedure== ==Notes== Please feel free to post comments, questions, or improvements to this protocol...</p> <p><b>New page</b></p><div>==Materials==<br /> <br /> *2.9 g NaCl<br /> *0.49 g MgSO4<br /> *25 mL 1 M Tris*Cl pH 7.5<br /> *BTV 500 mL dH2O<br /> <br /> ==Procedure==<br /> <br /> ==Notes==<br /> Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!<br /> <br /> &lt;!-- Please sign your name to your note by adding &lt;font face=&quot;courier&quot;&gt;&lt;nowiki&gt;'''*~~~~''':&lt;/nowiki&gt;&lt;/font&gt; to the beginning of your tip.--&gt;<br /> <br /> ==References==<br /> &lt;!-- If this protocol has papers or books associated with it, list those references here.--&gt;<br /> <br /> ==Contact==<br /> *Who has experience with this protocol?<br /> <br /> or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br /> <br /> &lt;!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --&gt;<br /> [[Category:Protocol]]<br /> &lt;!-- Move the relevant categories above this line to tag your protocol with the label<br /> [[Category:Protocol]]<br /> <br /> [[Category:Needs attention]]<br /> <br /> [[Category:In vitro]]<br /> <br /> [[Category:In vivo]]<br /> <br /> [[Category:In silico]]<br /> <br /> [[Category:DNA]]<br /> <br /> [[Category:RNA]]<br /> <br /> [[Category:Protein]]<br /> <br /> [[Category:Chemical]]<br /> <br /> [[Category:Escherichia coli]]<br /> <br /> [[Category:Yeast]]<br /> --&gt;</div> Wed, 30 May 2012 17:41:56 GMT Jacqueline K. Grimm http://openwetware.org/wiki/Talk:IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation