IGEM:Virginia 2012/Protocols/SM Buffer Preparation

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(New page: ==Materials== *2.9 g NaCl *0.49 g MgSO4 *25 mL 1 M Tris*Cl pH 7.5 *BTV 500 mL dH2O ==Procedure== ==Notes== Please feel free to post comments, questions, or improvements to this protocol...)
Current revision (09:40, 26 July 2012) (view source)
 
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*2.9 g NaCl
*2.9 g NaCl
*0.49 g MgSO4
*0.49 g MgSO4
-
*25 mL 1 M Tris*Cl pH 7.5
+
*25 mL 1 M Tris*Cl at pH 7.5
-
*BTV 500 mL dH2O
+
*less than 500 mL of distilled H2O
==Procedure==
==Procedure==
 +
* Add the NaCl and MgSO4 to a 1 L bottle.
 +
*  Add in 25 mL of 7.5 pH Tris-Cl
 +
* Bring this to a volume of 500 mL by adding distilled water.
 +
* Autoclave (large liquid).
==Notes==
==Notes==

Current revision

Contents

Materials

  • 2.9 g NaCl
  • 0.49 g MgSO4
  • 25 mL 1 M Tris*Cl at pH 7.5
  • less than 500 mL of distilled H2O

Procedure

  • Add the NaCl and MgSO4 to a 1 L bottle.
  • Add in 25 mL of 7.5 pH Tris-Cl
  • Bring this to a volume of 500 mL by adding distilled water.
  • Autoclave (large liquid).

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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