IGEM:Virginia 2012/Protocols/SM Buffer Preparation: Difference between revisions
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(New page: ==Materials== *2.9 g NaCl *0.49 g MgSO4 *25 mL 1 M Tris*Cl pH 7.5 *BTV 500 mL dH2O ==Procedure== ==Notes== Please feel free to post comments, questions, or improvements to this protocol...) |
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*2.9 g NaCl | *2.9 g NaCl | ||
*0.49 g MgSO4 | *0.49 g MgSO4 | ||
*25 mL 1 M Tris*Cl pH 7.5 | *25 mL 1 M Tris*Cl at pH 7.5 | ||
* | *less than 500 mL of distilled H2O | ||
==Procedure== | ==Procedure== | ||
* Add the NaCl and MgSO4 to a 1 L bottle. | |||
* Add in 25 mL of 7.5 pH Tris-Cl | |||
* Bring this to a volume of 500 mL by adding distilled water. | |||
* Autoclave (large liquid). | |||
==Notes== | ==Notes== | ||
Latest revision as of 06:40, 26 July 2012
Materials
- 2.9 g NaCl
- 0.49 g MgSO4
- 25 mL 1 M Tris*Cl at pH 7.5
- less than 500 mL of distilled H2O
Procedure
- Add the NaCl and MgSO4 to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
