http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&feed=atom&action=history 2013-05-19T10:54:24Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=605021&oldid=prev 2012-05-31T20:59:31Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:59, 31 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 66:</td> <td colspan="2" class="diff-lineno">Line 66:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Transfer to a new tube.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add an equal volume of [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/PEG/NaCl_Preparation PEG/NaCl] (probably 20 mL) to the conical tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add an equal volume of [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/PEG/NaCl_Preparation PEG/NaCl] (probably 20 mL) to the conical tube.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"># Vortex the tube.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <!-- diff generator: internal 2013-05-19 04:39:49 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:604808:newid:605021 --> </table>

Jacqueline K. Grimm http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604808&oldid=prev 2012-05-30T17:40:52Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:40, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 69:</td> <td colspan="2" class="diff-lineno">Line 69:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Discard supernatant in the sink. Let tube sit upside-down for 10-15 minutes to dry. Resuspend the pellet in <del class="diffchange diffchange-inline">500μl </del>of [http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation SM buffer]. Transfer to a 1.5 mL tube.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Discard supernatant in the sink. Let tube sit upside-down for 10-15 minutes to dry. Resuspend the pellet in <ins class="diffchange diffchange-inline">500 μL </ins>of [http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation SM buffer]. Transfer to a 1.5 mL tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant (10 krpm, 10 min)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant (10 krpm, 10 min)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Alex Zorychta http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604807&oldid=prev 2012-05-30T17:39:57Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:39, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 69:</td> <td colspan="2" class="diff-lineno">Line 69:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Discard supernatant in the sink. Let tube sit upside-down for 10-15 minutes to dry. Resuspend the pellet in 500μl of SM buffer. Transfer to a 1.<del class="diffchange diffchange-inline">5ml </del>tube.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Discard supernatant in the sink. Let tube sit upside-down for 10-15 minutes to dry. Resuspend the pellet in 500μl of <ins class="diffchange diffchange-inline">[http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/SM_Buffer_Preparation </ins>SM buffer<ins class="diffchange diffchange-inline">]</ins>. Transfer to a 1.<ins class="diffchange diffchange-inline">5 mL </ins>tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant (10 krpm, 10 min)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant (10 krpm, 10 min)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Alex Zorychta http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604800&oldid=prev 2012-05-30T17:35:42Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:35, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 66:</td> <td colspan="2" class="diff-lineno">Line 66:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Add an equal volume of [http://openwetware.org/wiki/IGEM:<del class="diffchange diffchange-inline">Virginia 2012</del>/Protocols/PEG/<del class="diffchange diffchange-inline">NaCl Preparation </del>PEG/NaCl] (probably 20 mL) to the conical tube.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Add an equal volume of [http://openwetware.org/wiki/IGEM:<ins class="diffchange diffchange-inline">Virginia_2012</ins>/Protocols/PEG/<ins class="diffchange diffchange-inline">NaCl_Preparation </ins>PEG/NaCl] (probably 20 mL) to the conical tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Alex Zorychta http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604798&oldid=prev 2012-05-30T17:35:15Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:35, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 66:</td> <td colspan="2" class="diff-lineno">Line 66:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the centrifuge and spin it down at 12krpm for 15 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Add an equal volume of PEG/NaCl (probably 20 mL) to the conical tube.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Add an equal volume of <ins class="diffchange diffchange-inline">[http://openwetware.org/wiki/IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation </ins>PEG/NaCl<ins class="diffchange diffchange-inline">] </ins>(probably 20 mL) to the conical tube.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Alex Zorychta http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604796&oldid=prev 2012-05-30T17:32:36Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:32, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 56:</td> <td colspan="2" class="diff-lineno">Line 56:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># If the O.D. (optical density) is less than 0.6 for the culture, continue incubating the flask until the O.D is around 0.6. When the O.D. is around 0.6, continue with the procedure below.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># If the O.D. (optical density) is less than 0.6 for the culture, continue incubating the flask until the O.D is around 0.6. When the O.D. is around 0.6, continue with the procedure below.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Using the <del class="diffchange diffchange-inline">C1V1</del>=<del class="diffchange diffchange-inline">C2V2 </del>formula, determine how much mitomycin C to add to the Erlenmeyer flask so that the final concentration is 2μg/ml. Pipette that amount of mitomycin C into the flask.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Using the <ins class="diffchange diffchange-inline">C&lt;sub&gt;1&lt;/sub&gt;V&lt;sub&gt;1&lt;/sub&gt;</ins>=<ins class="diffchange diffchange-inline">C&lt;sub&gt;2&lt;/sub&gt;V&lt;sub&gt;2&lt;/sub&gt; </ins>formula, determine how much mitomycin C to add to the Erlenmeyer flask so that the final concentration is 2μg/ml. Pipette that amount of mitomycin C into the flask.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Continue incubating the Erlenmeyer flask for 3 hours at 37°C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Continue incubating the Erlenmeyer flask for 3 hours at 37°C.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 74:</td> <td colspan="2" class="diff-lineno">Line 74:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place Falcon tube in oscillating incubator for 5-6 hours</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place Falcon tube in oscillating incubator for 5-6 hours</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Perform a plaque assay to determine the [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Plaque_Assay phage titer].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Perform a plaque assay to determine the [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Plaque_Assay phage titer].</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Alex Zorychta http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604778&oldid=prev 2012-05-30T16:45:10Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 16:45, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 62:</td> <td colspan="2" class="diff-lineno">Line 62:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># To find the amount of chloroform to add, divide the volume of the culture in the Erlenmeyer flask by <del class="diffchange diffchange-inline">125</del>. Pipette this amount of chloroform into the Erlenmeyer flask.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># To find the amount of chloroform to add, divide the volume of the culture in the Erlenmeyer flask by <ins class="diffchange diffchange-inline">25</ins>. Pipette this amount of chloroform into the Erlenmeyer flask.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Return the Erlenmeyer flask to the incubator for a few minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Return the Erlenmeyer flask to the incubator for a few minutes.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pour culture in Erlenmeyer flask into a conical tube.</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 69:</td> <td colspan="2" class="diff-lineno">Line 69:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Put the conical tube in the 4°C freezer and store overnight (or over the weekend).</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Take the conical tube to the centrifuge and spin it down at 12k rpm for 15 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Resuspend the pellet in 500μl of SM buffer.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Discard supernatant in the sink. Let tube sit upside-down for 10-15 minutes to dry. </ins>Resuspend the pellet in 500μl of SM buffer<ins class="diffchange diffchange-inline">. Transfer to a 1.5ml tube</ins>.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Spin down, and collect the supernatant <ins class="diffchange diffchange-inline">(10 krpm, 10 min)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Prepare a 3 mL liquid culture using the plate that was already streaked and the procedure for Part II.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place Falcon tube in oscillating incubator for 5-6 hours</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place Falcon tube in oscillating incubator for 5-6 hours</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Joseph J. Muldoon http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604718&oldid=prev 2012-05-30T03:27:18Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 03:27, 30 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Below is the procedure for producing a new phage suspension from <del class="diffchange diffchange-inline">a </del>bacteria that has the phage genome integrated. &nbsp;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Below is the procedure for producing a new phage suspension from bacteria that has the phage genome integrated. &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Part I'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Part I'''</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Agar plate (+ <del class="diffchange diffchange-inline">chloroamphenicol</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Agar plate (+ <ins class="diffchange diffchange-inline">chloramphenicol</ins>)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Plastic loop</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Plastic loop</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 23:</td> <td colspan="2" class="diff-lineno">Line 23:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Chloroform</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Chloroform</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Conical tube</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Conical tube</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*PEG/NaCl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">20% </ins>PEG/ <ins class="diffchange diffchange-inline">2.5M </ins>NaCl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*SM buffer</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*SM buffer</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Joseph J. Muldoon http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604533&oldid=prev 2012-05-28T18:23:15Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:23, 28 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Part I'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Part I'''</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Agar plate</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Agar plate <ins class="diffchange diffchange-inline">(+ chloroamphenicol)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Plastic loop</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Plastic loop</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Jacqueline K. Grimm http://openwetware.org/index.php?title=IGEM:Virginia_2012/Protocols/Phage_Amplification&diff=604531&oldid=prev 2012-05-28T17:13:44Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:13, 28 May 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 59:</td> <td colspan="2" class="diff-lineno">Line 59:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Continue incubating the Erlenmeyer flask for 3 hours at 37°C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Continue incubating the Erlenmeyer flask for 3 hours at 37°C.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Part III. Isolating the phage.'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Phage_Isolation </ins>'''Part III. Isolating the phage.'''<ins class="diffchange diffchange-inline">]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Get the Erlenmeyer flask from the incubator.</div></td></tr> <!-- diff generator: internal 2013-05-19 10:54:24 --> </table>

Jacqueline K. Grimm