IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation - Revision history

Jacqueline K. Grimm at 18:09, 30 May 2012

2012-05-30T18:09:47Z

←Older revision		Revision as of 18:09, 30 May 2012
Line 12:		Line 12:
	# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).		# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
	# Measure 58.4g NaCl and add it slowly.		# Measure 58.4g NaCl and add it slowly.
		+	# Autoclave (large liquid).
		+	# Stir it on the magnetic mixer again.

	==Notes==		==Notes==

Jacqueline K. Grimm at 17:38, 30 May 2012

2012-05-30T17:38:49Z

←Older revision		Revision as of 17:38, 30 May 2012
Line 10:		Line 10:

	# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.		# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
-	# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add ~~20g four times or 40g twice~~).	+	# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
	# Measure 58.4g NaCl and add it slowly.		# Measure 58.4g NaCl and add it slowly.

Alex Zorychta: /* Procedure */

2012-05-30T17:38:08Z

Procedure

←Older revision		Revision as of 17:38, 30 May 2012
Line 10:		Line 10:

	# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.		# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
-	# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g ~~three~~ times).	+	# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g four times or 40g twice).
	# Measure 58.4g NaCl and add it slowly.		# Measure 58.4g NaCl and add it slowly.

Jacqueline K. Grimm: New page: ==Materials== 20% PEG-8000 2.5 M NaCl milliQ H2O 1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...

2012-05-30T17:35:38Z

New page: ==Materials== *20% PEG-8000 *2.5 M NaCl *milliQ H2O *1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...

New page

==Materials==

*20% PEG-8000
*2.5 M NaCl
*milliQ H2O
*1 L bottle
*Stir bar

==Procedure==

# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g three times).
# Measure 58.4g NaCl and add it slowly.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!



==References==


==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]

IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation - Revision history

Jacqueline K. Grimm at 18:09, 30 May 2012

Jacqueline K. Grimm at 17:38, 30 May 2012

Alex Zorychta: /* Procedure */

Jacqueline K. Grimm: New page: ==Materials== *20% PEG-8000 *2.5 M NaCl *milliQ H2O *1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...

Jacqueline K. Grimm: New page: ==Materials== 20% PEG-8000 2.5 M NaCl milliQ H2O 1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...