IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation: Difference between revisions
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(New page: ==Materials== *20% PEG-8000 *2.5 M NaCl *milliQ H2O *1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...) |
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Revision as of 10:35, 30 May 2012
Materials
- 20% PEG-8000
- 2.5 M NaCl
- milliQ H2O
- 1 L bottle
- Stir bar
Procedure
- Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
- Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g three times).
- Measure 58.4g NaCl and add it slowly.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
