IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation

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(New page: ==Materials== *20% PEG-8000 *2.5 M NaCl *milliQ H2O *1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...)
(Procedure)
Line 10: Line 10:
# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
-
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g three times).
+
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g four times or 40g twice).
# Measure 58.4g NaCl and add it slowly.
# Measure 58.4g NaCl and add it slowly.

Revision as of 12:38, 30 May 2012

Contents

Materials

  • 20% PEG-8000
  • 2.5 M NaCl
  • milliQ H2O
  • 1 L bottle
  • Stir bar

Procedure

  1. Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
  2. Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g four times or 40g twice).
  3. Measure 58.4g NaCl and add it slowly.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

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