IGEM:Virginia 2012/Protocols/Bordet Gengou (BG) Agar Plate Preparation - Revision history

Jacqueline K. Grimm at 16:27, 1 June 2012

2012-06-01T16:27:27Z

←Older revision		Revision as of 16:27, 1 June 2012
Line 1:		Line 1:
-	This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used ~~for optimizing the isolation of~~ ''Bordatella''.	+	This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates], a differential medium used to isolate ''Bordatella''.

	==Materials==		==Materials==

Alex Zorychta: /* Materials */

2012-05-31T18:54:41Z

Materials

←Older revision		Revision as of 18:54, 31 May 2012
Line 9:		Line 9:
	* Stir Bar		* Stir Bar
	* 100 mL screw cap bottles		* 100 mL screw cap bottles
		+	* 13 mL defibrinated sheep blood
	* Autoclave		* Autoclave
	* Autoclave tape		* Autoclave tape

Alex Zorychta at 18:06, 31 May 2012

2012-05-31T18:06:40Z

←Older revision		Revision as of 18:06, 31 May 2012
Line 1:		Line 1:
	This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used for optimizing the isolation of ''Bordatella''.		This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used for optimizing the isolation of ''Bordatella''.
-
-	~~<html><img src="Image:https://fscimage.fishersci.com/images/F103719~wl.jpg"></html>~~

	==Materials==		==Materials==

Alex Zorychta at 18:03, 31 May 2012

2012-05-31T18:03:16Z

←Older revision		Revision as of 18:03, 31 May 2012
Line 1:		Line 1:
	This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used for optimizing the isolation of ''Bordatella''.		This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used for optimizing the isolation of ''Bordatella''.
		+
		+	<html><img src="Image:https://fscimage.fishersci.com/images/F103719~wl.jpg"></html>

	==Materials==		==Materials==

Alex Zorychta at 17:59, 31 May 2012

2012-05-31T17:59:59Z

←Older revision		Revision as of 17:59, 31 May 2012
Line 1:		Line 1:
		+	This protocol makes the [http://en.wikipedia.org/wiki/Bordet-Gengou_agar Bordet Gengou Agar Plates] used for optimizing the isolation of ''Bordatella''.
		+
	==Materials==		==Materials==

Alex Zorychta: /* Materials */

2012-05-31T17:57:23Z

Materials

←Older revision		Revision as of 17:57, 31 May 2012
Line 9:		Line 9:
	* Autoclave		* Autoclave
	* Autoclave tape		* Autoclave tape
-	* ~~4<sup>o</sup>C~~ Chamber	+	* 4°C Chamber

	==Procedure==		==Procedure==

Alex Zorychta: /* Procedure */

2012-05-31T17:57:04Z

Procedure

←Older revision		Revision as of 17:57, 31 May 2012
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	* Leave caps loose enough for air exchange and place autoclave tape between cap and bottle.		* Leave caps loose enough for air exchange and place autoclave tape between cap and bottle.
	* Autoclave		* Autoclave
-	* Tighten the caps and store at ~~4<sup>o</sup>C~~ when done.	+	* Tighten the caps and store at 4°C when done.

	===Pour plates===		===Pour plates===
Line 28:		Line 28:
	* ''Note: for antibiotic plates, add 1 mL of 100x solution of antibiotic at time blood is added.''		* ''Note: for antibiotic plates, add 1 mL of 100x solution of antibiotic at time blood is added.''
	* When plates have cooled and become solid, '''turn them upside down'''.		* When plates have cooled and become solid, '''turn them upside down'''.
-	* Leave overnight to reduce condensation, then package, date, label, and store at ~~4<sup>o</sup>C~~.	+	* Leave overnight to reduce condensation, then package, date, label, and store at 4°C.

	==Notes==		==Notes==

Alex Zorychta: New page: ==Materials== * 30g of Bordet Gengou Agar Base * 1 L of distilled H₂O * 13 mL glycerol * 2600 mL Fernback Flask * Stir Bar * 100 mL screw cap bottles * Autoclave * Autoclave ta...

2012-05-31T17:54:40Z

New page: ==Materials== * 30g of Bordet Gengou Agar Base * 1 L of distilled H2O * 13 mL glycerol * 2600 mL Fernback Flask * Stir Bar * 100 mL screw cap bottles * Autoclave * Autoclave ta...

New page

==Materials==

* 30g of Bordet Gengou Agar Base
* 1 L of distilled H2O
* 13 mL glycerol
* 2600 mL Fernback Flask
* Stir Bar
* 100 mL screw cap bottles
* Autoclave
* Autoclave tape
* 4oC Chamber

==Procedure==

===Making solution===
* Add 30g of Bordet Gengou Agar Base to 1 liter of distilled H2O in a 2600 mL Fernback Flask while stirring.
* Turn on heat to '''medium hot'''.
* Add 13 mL of glycerol
* When thoroughly heated and in solution, aliquot 80 mL in to 100 mL screw cap bottles.
* Leave caps loose enough for air exchange and place autoclave tape between cap and bottle.
* Autoclave
* Tighten the caps and store at 4oC when done.

===Pour plates===
* Heat autoclaved BG agar bottles in hot water bath until agar is completely liquid.
* This requires hottest setting on hot plate. Remove bottles to cool on bench top.
* When comfortable to touch, add 13 mL defibrinated sheep blood and pour into 60 x 15 mm culture dishes, '''avoiding air bubbles'''. ''(If blood is added when BG agar is too hot, it will hemolyze the blood, turning it brown)''.
* ''Note: for antibiotic plates, add 1 mL of 100x solution of antibiotic at time blood is added.''
* When plates have cooled and become solid, '''turn them upside down'''.
* Leave overnight to reduce condensation, then package, date, label, and store at 4oC.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!



==References==


==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]

IGEM:Virginia 2012/Protocols/Bordet Gengou (BG) Agar Plate Preparation - Revision history

Jacqueline K. Grimm at 16:27, 1 June 2012

Alex Zorychta: /* Materials */

Alex Zorychta at 18:06, 31 May 2012

Alex Zorychta at 18:03, 31 May 2012

Alex Zorychta at 17:59, 31 May 2012

Alex Zorychta: /* Materials */

Alex Zorychta: /* Procedure */

Alex Zorychta: New page: ==Materials== * 30g of Bordet Gengou Agar Base * 1 L of distilled H2O * 13 mL glycerol * 2600 mL Fernback Flask * Stir Bar * 100 mL screw cap bottles * Autoclave * Autoclave ta...

Alex Zorychta: New page: ==Materials== * 30g of Bordet Gengou Agar Base * 1 L of distilled H₂O * 13 mL glycerol * 2600 mL Fernback Flask * Stir Bar * 100 mL screw cap bottles * Autoclave * Autoclave ta...