IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 9/16/12</span> | ||
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== | ==Week of 9/16/12== | ||
Performed gel extraction of ligated backbones (both E+P and E+S); failed to show up in | |||
adequate amounts when nanodropped. | |||
<br> | |||
Ligated Plasmid+RBS (E+S), SynCG (X+P), and RFP (X+P) at 3:1 and 1:1 ratios of both | |||
2X ligase buffer and promega DNA ligase and performed PCR. | |||
<br> | |||
Repeated ligation and performed PCR purification: P+RBS+RFP and P+RBS+SynCG | |||
demonstrate proper amounts of DNA but at low concentrations, suggesting that perhaps | |||
the DNA is not the intended amplicon. | |||
<br> | |||
Discovered that roughly half of the previous pregnancy tests now appear positive, | |||
brighter than evaporation lines would be. Some tests for 5 mM DTT (denaturing | |||
solution), 0.5 mM DTT, 0.05 mM DTT, and 0 mM DTT are now positive with the | |||
majority in the latter two groups. | |||
<br> | |||
Nanodropped the P+RBS+RFP, P+RBS+SynCG, backbone (E+S), backbone (E+P), | |||
and SynCG all (E+P): only the backbone (E+S) and SynCG all (E+P) showing proper | |||
concentrations. The latter includes the IDT plasmid backbone. | |||
<br> | |||
Plated the bbp9 biobricks (both 3:1 and 1:1 ratios), the hCG biobrick (in 3:1, 1:1, and | |||
1:3 ratios), backbone, original BBa_K331022, and a DNA lacking group, producing | |||
thousands of cells on each plate (except for the last), demonstrating that many of the cells | |||
have empty vectors. | |||
Revision as of 17:07, 3 October 2012
Week of 9/16/12 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Week of 9/16/12Performed gel extraction of ligated backbones (both E+P and E+S); failed to show up in
adequate amounts when nanodropped.
|