IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/28

From OpenWetWare
Jump to navigationJump to search
UIUC - Arsenic Collection System! <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

8.28.10

gel

Lane Sample Amount
1 1 Kb Ladder 1 μL
2 ArsB1 5 μL sample + 1 μL dye
3 ArsB2 5 μL sample + 1 μL dye
4 ArsB3 5 μL sample + 1 μL dye
5 ArsB4 5 μL sample + 1 μL dye
6 Negative control 5 μL sample + 1 μL dye

Overnight of ArsRB0015 was red, will try using a different backbone next time

11:30 AM

made three 5 mL cultures of ArsRSDM2, grew overnight at 30°C

Streaked MG1655 on LB from glycerol stock to use in genome prep later next week.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:University_of_Illinois_Urbana_Champaign/2009/Notebook/Bioware_2010_Arsenic_Bioremediation/2010/08/28&oldid=449468"