IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/20
 UIUC - Arsenic Collection System!
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7/20/1010:50 AM checked plates from yesterday, got lawns. inoculated 5 mL borth for each plate, incubating in 37°C room until tonight. Tomorrow will miniprep/PCR to see if insert actually in plasmid. Also made new Kan plates and will replate on these since there really shouldn't have been lawns this morning. 11:30 AM PCR clean up of yesterday's samples: pRpoS 438 30.5 ng/μL ArsR 25 ng/μL pArsR 25.5 ng/μL 2:35 PM Digestions ArsR
pSB1A2
incubate at 37°C for 15 min, inactivate at 80°C for 20 min. 3:15 PM Ligations ArsR-pSB1A2
incubate at room temp. for 10 min, inactivate at 80°C for 20 min. 4:15 PM Transformations 100 μL cells, 3 μL ligation product. electroporate, add 1 mL LB, recover at 37°C for 1 hour. 100 μL NEB cells, 3 μL ligation product. electroporate, add 1 mL LB, recover at 37°C for 1 hour. 5:15 PM Plating 200 ul on Amp plate
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