IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/10
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Colonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking.