IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/09/03 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
(Entry title)
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
-
==Entry title==
+
==Floor One==
-
* Insert your content here.
+
Made 1% agarose gel.<br>
 +
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br>
 +
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products.<br>
 +
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br>
 +
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br>
 +
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.

Revision as of 06:45, 25 September 2013

iGEM Project name 1 Main project page
Previous entry      Next entry

Floor One

Made 1% agarose gel.
Filtered, concentrated and resuspended spore stocks in 20% glycerol.
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products.
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.



Personal tools