IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03
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Made 1% agarose gel.
Filtered, concentrated and resuspended spore stocks in 20% glycerol.
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products fig 1 found that there were two bands in addition to the undigested plasmid. Unsure what the middle band could be.
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays (examples in fig 2). A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.
Fig 2. Bioassay plates showing two drops of spore stock (left is sample 38.3^-4 and right is sample 24.6^-6) which has been challenged with SNA containing C.albicans.