IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03

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(Floor One)
(Floor One)
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Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br>
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br>
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays (examples in ''fig 2''). A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays (examples in ''fig 2''). A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.
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[[Image:bioassays.png]] Fig 2. Bioassay plates showing two drops of spore stock (left is sample 38.3^-4 and right is sample 24.6^-6) which has been challenged with SNA containing ''C.albicans''.
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[[Image:bioassays.png]]  
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Fig 2. Bioassay plates showing two drops of spore stock (left is sample 38.3^-4 and right is sample 24.6^-6) which has been challenged with SNA containing ''C.albicans''.

Revision as of 18:15, 29 September 2013

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Floor One

Fig 1. Gel image of the digested pMS82. Lane 1 and 2 are 1Kb+ ladders, and lanes 3 and 4 are the digested plasmid. Lane 5 is a control of undigested pMS82. The lower band in lanes 3 and 4 is the excised neomycin gene, but the band above that is unknown.
Fig 1. Gel image of the digested pMS82. Lane 1 and 2 are 1Kb+ ladders, and lanes 3 and 4 are the digested plasmid. Lane 5 is a control of undigested pMS82. The lower band in lanes 3 and 4 is the excised neomycin gene, but the band above that is unknown.

Made 1% agarose gel.
Filtered, concentrated and resuspended spore stocks in 20% glycerol.
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products fig 1 found that there were two bands in addition to the undigested plasmid. Unsure what the middle band could be.
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays (examples in fig 2). A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N. Image:bioassays.png Fig 2. Bioassay plates showing two drops of spore stock (left is sample 38.3^-4 and right is sample 24.6^-6) which has been challenged with SNA containing C.albicans.



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