IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03

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(Floor One)
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==Floor One==
==Floor One==
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Made 1% agarose gel.<br>
Made 1% agarose gel.<br>
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br>
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br>
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Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products.<br>
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Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products ''fig 1''.<br>
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br>
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br>
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br>
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br>

Revision as of 11:48, 29 September 2013

iGEM Project name 1 Main project page
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Floor One

Made 1% agarose gel.
Filtered, concentrated and resuspended spore stocks in 20% glycerol.
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products fig 1.
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.



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