IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/21

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(Autocreate 2013/08/21 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Twelve</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Floor Two==
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* Insert your content here.
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Minipreps were prepared using 5ml from each overnight culture and incubated with PstI restriction enzyme for analysis by gel electrophoresis. We also prepared the biobrick BBa_K1041000 by cutting with restriction enzyme NdeI and analysing on an agarose gel. The large fragment was cut from the gel to be purified. This process should remove the extra NdeI sites that were introduced to the sequence.<br>
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100μL from two of the overnight cultures was used to inoculate two fresh 10ml LB with added antibiotics Kanamycin and Chlorophenicol. They were incubated for 2 hours at 37°C and then one culture was induced with IPTG.

Revision as of 06:31, 21 August 2013

Week Twelve Main project page
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Floor Two

Minipreps were prepared using 5ml from each overnight culture and incubated with PstI restriction enzyme for analysis by gel electrophoresis. We also prepared the biobrick BBa_K1041000 by cutting with restriction enzyme NdeI and analysing on an agarose gel. The large fragment was cut from the gel to be purified. This process should remove the extra NdeI sites that were introduced to the sequence.
100μL from two of the overnight cultures was used to inoculate two fresh 10ml LB with added antibiotics Kanamycin and Chlorophenicol. They were incubated for 2 hours at 37°C and then one culture was induced with IPTG.



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