IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/30

From OpenWetWare

Jump to: navigation, search
Current revision (13:33, 22 August 2013) (view source)
(Floor 2)
 
Line 14: Line 14:
==Floor 2==
==Floor 2==
-
Minipreps of grown cultures overnight were prepared, then samples were cut by (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. After incubation samples were analysed using agarose gel electrophoresis.
+
Minipreps of grown cultures overnight were prepared, then samples were cut by (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. After incubation samples were analysed using agarose gel electrophoresis ''fig 2''.
-
[[Image:30 july.JPG|thumb| Restriction digest reactions using (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. Lanes 1 and 7 are sample 1 colony 1, Lanes  2 and 8 are sample 1 colony 2. Lanes 3 and 9 are sample 2 colony 1, lanes 4 and 10 are sample 2 colony 2]]
+
[[Image:30 july.JPG|thumb|Fig 2: Restriction digest reactions using (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. Lanes 1 and 7 are sample 1 colony 1, Lanes  2 and 8 are sample 1 colony 2. Lanes 3 and 9 are sample 2 colony 1, lanes 4 and 10 are sample 2 colony 2]]

Current revision

Week Nine Main project page
Previous entry      Next entry

Floor 1

Made up 8 l SFM agar media + 250 ml LB (-NaCl) broth.

Streaked plates - lawns of single Streptomyces colonies that were sporulating, streak purifications of contaminated plates and isolations of single Streptomyces colonies found on diluted soil sample plates (39-60).

Streaked S4 strains (ex-conjugation) onto SFM media + Apr (as contains pAU3-45).

Floor 2

Minipreps of grown cultures overnight were prepared, then samples were cut by (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. After incubation samples were analysed using agarose gel electrophoresis fig 2.

Fig 2: Restriction digest reactions using (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. Lanes 1 and 7 are sample 1 colony 1, Lanes  2 and 8 are sample 1 colony 2. Lanes 3 and 9 are sample 2 colony 1, lanes 4 and 10 are sample 2 colony 2
Fig 2: Restriction digest reactions using (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. Lanes 1 and 7 are sample 1 colony 1, Lanes 2 and 8 are sample 1 colony 2. Lanes 3 and 9 are sample 2 colony 1, lanes 4 and 10 are sample 2 colony 2



Personal tools