IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25
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The membrane was retrieved from the shaker and the blocking solution used was stored in the fridge. The membrane was washed with TBST and TBS solutions respectively for ten minutes. The membrane was incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.