IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/19

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Also transformations were done on the plasmids in order to make more of the DNA.
Also transformations were done on the plasmids in order to make more of the DNA.
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===Floor 1===
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Numbered and labelled further samples collected (39-51).
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Made 6 x 500 ml flasks of SFM, autoclaved and poured into plates to set (25 ml each).
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Colony streaking - some plates need longer to grow as no Strep. has appeared or the colonies arent sporulating yet. Streaked all Strep.-like colonies and interesting looking colonies.
==Outreach==
==Outreach==

Revision as of 13:37, 22 July 2013

Week Seven Main project page
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Lab

Floor 2

Following the sequencing analysis from yesterday, we decided to do restriction enzyme digest reactions for our AntGp-Neo biobrick to fix the duplicated Xba1 sites, so that they get removed. Samples were run on a gel and fragments were cut and purified, DNA was stored in the freezer.

Also transformations were done on the plasmids in order to make more of the DNA.

Floor 1

Numbered and labelled further samples collected (39-51).

Made 6 x 500 ml flasks of SFM, autoclaved and poured into plates to set (25 ml each).

Colony streaking - some plates need longer to grow as no Strep. has appeared or the colonies arent sporulating yet. Streaked all Strep.-like colonies and interesting looking colonies.

Outreach

An excel sheet containing all the details about the soil and sediment samples was updated.



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