IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/18

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(Floor Two)
Current revision (08:38, 25 September 2013) (view source)
(Floor One)
 
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Sequencing results arrived and we spent the time analysing them. We confirmed one of our bio ricks which is the J04450 with Nde1 site inserted. But the other bio brick had a problem where 3 Xba1 sites were inserted instead of one. Therefore, we planned experiments to fix this error over next week.
Sequencing results arrived and we spent the time analysing them. We confirmed one of our bio ricks which is the J04450 with Nde1 site inserted. But the other bio brick had a problem where 3 Xba1 sites were inserted instead of one. Therefore, we planned experiments to fix this error over next week.
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The supernatant and resuspended pellets from the protein expression were analysed using a 10% SDS Polyacrylamide gel which was then stained with Coomassie blue.
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The insoluble and soluble fractions from the protein expression were analysed using a 10% SDS Polyacrylamide gel which was then stained with Coomassie blue.
===Floor One===
===Floor One===
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Observed previously plated samples (1-38) - most showed at least one example of Strep (!!!) - small white colonys with brown ring around. Sporulating colonies have a grey centre.
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Observed previously plated samples (1-38) - most showed at least one example of <i>Streptomyces</i> sp. or another Actinomycete - often small white colonies with a surrounding brown ring of secondary metabolite production. Sporulating colonies have a grey centre.
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Re-plated samples that showed no Strep. growth. Dilutions of 10^-2, 10^-3 and 10^-4 were plated.
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Re-plated samples that showed no <i>Streptomyces</i> growth. Dilutions of 10<sup>-2</sup>, 10<sup>-3</sup> and 10<sup>-4</sup> were plated.
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Transformations - growth on all plates = successful transformations, now have resistance to antibiotic in media. Only diluted samples are useful (single colonies), in the un-diluted samples the colonies have merged.
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Growth occurred on all the transformation plates indicating successful transformations. Only the plated diluted samples are useful as they show clear single colonies whereas colonies have merged on the undiluted plates.
==Outreach==
==Outreach==

Current revision

Week Seven Main project page
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Lab

Floor Two

Sequencing results arrived and we spent the time analysing them. We confirmed one of our bio ricks which is the J04450 with Nde1 site inserted. But the other bio brick had a problem where 3 Xba1 sites were inserted instead of one. Therefore, we planned experiments to fix this error over next week.

The insoluble and soluble fractions from the protein expression were analysed using a 10% SDS Polyacrylamide gel which was then stained with Coomassie blue.

Floor One

Observed previously plated samples (1-38) - most showed at least one example of Streptomyces sp. or another Actinomycete - often small white colonies with a surrounding brown ring of secondary metabolite production. Sporulating colonies have a grey centre.

Re-plated samples that showed no Streptomyces growth. Dilutions of 10-2, 10-3 and 10-4 were plated.

Growth occurred on all the transformation plates indicating successful transformations. Only the plated diluted samples are useful as they show clear single colonies whereas colonies have merged on the undiluted plates.

Outreach

The team was split into two groups and went for collecting sediment samples from around Norfolk.




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