IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/15

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(Floor One)
(Floor One)
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All soil samples received so far were numbered and decanted into uniform tubes (1-38).
All soil samples received so far were numbered and decanted into uniform tubes (1-38).
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Samples 1-38 were diluted from 10^-1 up to 10^-10.  
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Samples 1-38 were diluted from 10<sup>-1</sup> up to 10<sup>-10</sup>.  
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Soya flour media (SFM) was made in quantities of 6 x 500 ml - agar, soya flour, mannitol and tap water. Autoclaved for 1 hour then poured into plates to set.
+
Soya flour mannitol (SFM) media was made in quantities of 6 x 500 ml, autoclaved for 1 hour then poured into plates to set.
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Dilutions of 10^-2, 10^-4, 10^-6, 10^-8 and 10^-10 of samples 1-11 were plated on SFM + 500 ul in 500 ml of Nystatin (Ny) and Cyclohexamide (Cy).  
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Dilutions of 10<sup>-2</sup>, 10<sup>-4</sup>, 10<sup>-6</sup>, 10<sup>-8</sup> and 10<sup>-10</sup> of samples 1-11 were plated onto SFM + 500 μL per 500 ml of Nystatin (Ny) and Cyclohexamide (Cy).  
Plates were incubated at 30° for 7 days.
Plates were incubated at 30° for 7 days.

Revision as of 07:30, 25 September 2013

Week Seven Main project page
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Lab

Floor Two

Further digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1.

(We were not supposed to use EcoR1 but there was no enough Xba1).

After incubation samples were run on a gel to analyse them.

Floor One

All soil samples received so far were numbered and decanted into uniform tubes (1-38).

Samples 1-38 were diluted from 10-1 up to 10-10.

Soya flour mannitol (SFM) media was made in quantities of 6 x 500 ml, autoclaved for 1 hour then poured into plates to set.

Dilutions of 10-2, 10-4, 10-6, 10-8 and 10-10 of samples 1-11 were plated onto SFM + 500 μL per 500 ml of Nystatin (Ny) and Cyclohexamide (Cy).

Plates were incubated at 30° for 7 days.

Outreach

Carried on e-mailing iGEm teams , but now focused on sediment after communications with Lab on floor 1.

Ben Thompson arrangements.

E-mailed iGEM contact (Nitwa ..) about the idea of an outreach registry, so that teams who are organizing a public engagement event (i.e. Forum) could have a infomrational resource pool to draw from, instead of starting from scrach each time.

Will follow up iGEM (Westminster) as they have a simliar project and may be interested in such a resource and collaborating in its construction.

Organized a meeting with Anne Osbourne (JIC) who spoke at this years SB6.0 SynBio conference. This is related to projects leading on from/inspired by iGEM and will focus on how iGEM bricks can be used in real research.



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