IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/15

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Floor One)
Line 19: Line 19:
All soil samples received so far were numbered and decanted into uniform tubes (1-38).
All soil samples received so far were numbered and decanted into uniform tubes (1-38).
-
Samples 1-38 were diluted by a factor of 10^-10.  
+
Samples 1-38 were diluted from 10^-1 up to 10^-10.  
Soya flour media (SFM) was made in quantities of 6 x 500 ml - agar, soya flour, mannitol and tap water. Autoclaved for 1 hour then poured into plates to set.
Soya flour media (SFM) was made in quantities of 6 x 500 ml - agar, soya flour, mannitol and tap water. Autoclaved for 1 hour then poured into plates to set.

Revision as of 11:30, 22 August 2013

Week Seven Main project page
Previous entry      Next entry

Lab

Floor Two

Further digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1.

(We were not supposed to use EcoR1 but there was no enough Xba1).

After incubation samples were run on a gel to analyse them.

Floor One

All soil samples received so far were numbered and decanted into uniform tubes (1-38).

Samples 1-38 were diluted from 10^-1 up to 10^-10.

Soya flour media (SFM) was made in quantities of 6 x 500 ml - agar, soya flour, mannitol and tap water. Autoclaved for 1 hour then poured into plates to set.

Dilutions of 10^-2, 10^-4, 10^-6, 10^-8 and 10^-10 of samples 1-11 were plated on SFM + 500 ul in 500 ml of Nystatin (Ny) and Cyclohexamide (Cy).

Plates were incubated at 30° for 7 days.

Outreach

Carried on e-mailing iGEm teams , but now focused on sediment after communications with Lab on floor 1.

Ben Thompson arrangements.

E-mailed iGEM contact (Nitwa ..) about the idea of an outreach registry, so that teams who are organizing a public engagement event (i.e. Forum) could have a infomrational resource pool to draw from, instead of starting from scrach each time.

Will follow up iGEM (Westminster) as they have a simliar project and may be interested in such a resource and collaborating in its construction.

Organized a meeting with Anne Osbourne (JIC) who spoke at this years SB6.0 SynBio conference. This is related to projects leading on from/inspired by iGEM and will focus on how iGEM bricks can be used in real research.



Personal tools