IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11

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Week Six Main project page
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Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol fig 2. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel fig 3to confirm the results.

Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.
Fig 3: Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.
Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities.
Fig 2:Cultures grown overnight after inoculation of 3 colonies from the J04450-Nde1 plate (3a,b,c), showing a pink colour with slightly different intensities.


The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.



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