IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29: Difference between revisions
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==Floor 2== | ==Floor 2== | ||
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel ''fig | Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel ''fig 1''. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. | ||
[[Image:29 july.JPG|thumb|Fig | [[Image:29 july.JPG|thumb|Fig 1:Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.]] | ||
Revision as of 11:33, 22 August 2013
 Week Nine
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Floor 1Streaked lawns of single Streptomyces colonies that appeared to be sporulating and had large clear single colonies. Re-streaked contaminated single Streptomyces colony plates. Streaked from the plates in the conjugated Steptomyces trial that had grown on the kanamycin plates (?). Floor 2Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel fig 1. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. 
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