IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/11: Difference between revisions
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Jessica Gray (talk | contribs) (Autocreate 2014/08/11 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Week 9 Day 1== | ||
- Picked 2 colonies of the level 2 dig-lig's transformed on friday. A, B, C, D, E, F, G and the level 2 Vector pAGM8031. Colonies grown in 1 ml of LB containing 1 μL/mL of Spectinomycin O/N. | |||
- Colony PCR of agro cultures grown for 2 days. Construct numbers 2, 3, 4, 10, 11. 4 cultures per construct were grown. Run on a 1% TAE gel with a positive control of pure DNA for each construct. | |||
Revision as of 06:35, 11 August 2014
 iGEM Project name 1
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Week 9 Day 1- Picked 2 colonies of the level 2 dig-lig's transformed on friday. A, B, C, D, E, F, G and the level 2 Vector pAGM8031. Colonies grown in 1 ml of LB containing 1 μL/mL of Spectinomycin O/N. - Colony PCR of agro cultures grown for 2 days. Construct numbers 2, 3, 4, 10, 11. 4 cultures per construct were grown. Run on a 1% TAE gel with a positive control of pure DNA for each construct.
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