IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/08: Difference between revisions

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(Autocreate 2014/08/08 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
==Week 8 Day 4==
* Insert your content here.
* Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.


* Plated end linkers 2 and 3 on ampicillin
* Picked colonies of ''Agrobacterium tumerfaciens'', 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.




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Revision as of 13:12, 8 August 2014

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Week 8 Day 4

  • Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.
  • Plated end linkers 2 and 3 on ampicillin
  • Picked colonies of Agrobacterium tumerfaciens, 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.
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