IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/24: Difference between revisions
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- DNA quantified using Nanodrop and blanked with EB buffer. | - DNA quantified using Nanodrop and blanked with EB buffer. | ||
- Further mini prep of O/N cultures grown on 22/07/14 and 23/07/14 from the BioBricks requested from the <a href="http://2010.igem.org/Team:Nevada">2010 Nevada team's</a> project 5 mL was analysed from an inoculation of 10 mL LB + CAM. | |||
Biobricks obtained are: | |||
BBa_K414001 | |||
BBa_K414003 | |||
BBa_K414004 | |||
BBa_K414008 | |||
They were run on a 1% agarose gel with TAE buffer. | |||
Revision as of 07:45, 24 July 2014
 iGEM Project name 1
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Week 6 Day 4- Mini-prep of O/N cultures grown on 23/07/14 of DNA synthesised by DNA 2.0. Tubes 1 to 6: 1. AtPR1 Conc: 182.2 ng/µl 260/280: 1.94 2. ArvBS3 Conc: 342.7 ng/µl 260/280: 1.93 3. NbBi1-RNA Conc: 152.2 ng/µl 260/280: 1.95 4. NbProB-RN Conc: 181.6 ng/µl 260/280: 1.90 5. AvrXa27 T Conc: 263.2 ng/µl 260/280: 1.90 6. OsXa27 Pro Conc: 286.5 ng/µl 260/280: 1.90 - DNA quantified using Nanodrop and blanked with EB buffer. - Further mini prep of O/N cultures grown on 22/07/14 and 23/07/14 from the BioBricks requested from the <a href="http://2010.igem.org/Team:Nevada">2010 Nevada team's</a> project 5 mL was analysed from an inoculation of 10 mL LB + CAM. Biobricks obtained are: BBa_K414001 BBa_K414003 BBa_K414004 BBa_K414008 They were run on a 1% agarose gel with TAE buffer.
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