IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/04: Difference between revisions
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(Autocreate 2014/07/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Week 3, Day 5== | ||
TSL | |||
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-Digest of PCR samples from 3.7.14 | |||
-Gel purification and quantification of digested fragments (now ready to ligate into PSB1C3 backbone) | |||
-Digest of PSB1C3 plasmid with EcoR1 and PST1 (removes blunt ends) | |||
-Dephosphorylation of plasmid backbone | |||
-Colony PCR of colonies picked from agro plates (transformed 10.7.14)to confirm that the DNA is what we want. | |||
Revision as of 08:02, 24 July 2014
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Week 3, Day 5TSL -Digest of PCR samples from 3.7.14 -Gel purification and quantification of digested fragments (now ready to ligate into PSB1C3 backbone) -Digest of PSB1C3 plasmid with EcoR1 and PST1 (removes blunt ends) -Dephosphorylation of plasmid backbone
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