IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/03: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Week 3, Day 4==


TSL
TSL
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- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA
- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA
  • Pro + 5UTR
 
  • CDS
• Pro + 5UTR
  • Ter + 3UTR
 
• CDS
 
• Ter + 3UTR


-Ran PCR product on a gel to check amplification
-Ran PCR product on a gel to check amplification
-PCR product purification using phenol chloroform
-PCR product purification using phenol chloroform


Latest revision as of 00:07, 27 September 2017

iGEM Project name 1 Main project page
Previous entry      Next entry

Week 3, Day 4

TSL

- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA

• Pro + 5UTR

• CDS

• Ter + 3UTR

-Ran PCR product on a gel to check amplification

-PCR product purification using phenol chloroform



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