IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/30: Difference between revisions
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Jessica Gray (talk | contribs) (Autocreate 2014/06/30 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
Jessica Gray (talk | contribs) |
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== | ==Week 3 Day 1== | ||
TSL | |||
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- Set up 10 Dig-Lig reactions with new BSA1 enzyme provided by TSL. | |||
- DNA was transformed into ''E.coli'' using electroporation method previously discussed. | |||
- Transformed bacteria was spreadplated onto canamycin plates containing XGAL and IPTG and incubated O/N at 37°C. | |||
- Mini-prep of DNA obtained from Week 2's successful Dig-Lig experiments according to Qiagen protocol. However, DNA eluted in 25 µl of eluting buffer rather than 50 µl as protocol suggests. | |||
- DNA conc. of each sample quanitifed using the Nanodrop. | |||
- DNA diluted to obtain 5 ng in each 5 µl sample. | |||
- Sense and Antisense primers to each 5 µl DNA (+H20) to achieve 20 µl sample in total and sent for sequencing to verify construct sequence. | |||
Revision as of 07:39, 22 July 2014
 iGEM Project name 1
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Week 3 Day 1TSL - Set up 10 Dig-Lig reactions with new BSA1 enzyme provided by TSL. - DNA was transformed into E.coli using electroporation method previously discussed. - Transformed bacteria was spreadplated onto canamycin plates containing XGAL and IPTG and incubated O/N at 37°C. - Mini-prep of DNA obtained from Week 2's successful Dig-Lig experiments according to Qiagen protocol. However, DNA eluted in 25 µl of eluting buffer rather than 50 µl as protocol suggests. - DNA conc. of each sample quanitifed using the Nanodrop. - DNA diluted to obtain 5 ng in each 5 µl sample. - Sense and Antisense primers to each 5 µl DNA (+H20) to achieve 20 µl sample in total and sent for sequencing to verify construct sequence.
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