IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/27: Difference between revisions

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(Autocreate 2014/06/27 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
Week 2 Day 4
* Insert your content here.




TSL


----
- Found poor blue/white selection on dig-ligs from 26.06.14. Suspected to be due to either the enzyme used or PCR block.
- '''Set up a new set of dig-ligs to test the source of this problem.'''
  • Li - block L, iGEM enzyme
  • Lmy - block L, MY enzyme
  • Bi - block B, iGEM enzyme
  • Bmy - block B, MY enzyme
- Transformed into E.coli using electroporation
  •
- Spread plate onto plates prepared with xGAL, IPTG
 
 
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Latest revision as of 00:07, 27 September 2017

iGEM Project name 1 Main project page
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Week 2 Day 4


TSL

- Found poor blue/white selection on dig-ligs from 26.06.14. Suspected to be due to either the enzyme used or PCR block. - Set up a new set of dig-ligs to test the source of this problem. 

 • Li - block L, iGEM enzyme
 • Lmy - block L, MY enzyme 
 • Bi - block B, iGEM enzyme 
 • Bmy - block B, MY enzyme

- Transformed into E.coli using electroporation 

 •

- Spread plate onto plates prepared with xGAL, IPTG



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