IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/26: Difference between revisions
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Jessica Gray (talk | contribs) (Autocreate 2014/06/26 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
Jessica Gray (talk | contribs) |
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== | == Week 2 Day 4== | ||
TSL | |||
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- Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies. | |||
- Selected 2 white colonies from each plated and colony PCR performed. | |||
- Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct. | |||
- Decided to repeat the 11 Dig-Lig experiments | |||
- Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands. | |||
Revision as of 07:29, 22 July 2014
 iGEM Project name 1
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Week 2 Day 4TSL - Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies. - Selected 2 white colonies from each plated and colony PCR performed. - Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct. - Decided to repeat the 11 Dig-Lig experiments - Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands.
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