IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | == Week 2 Day 4== | ||
TSL | |||
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- Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies. | |||
- Selected 2 white colonies from each plated and colony PCR performed. | |||
- Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct. | |||
- Decided to repeat the 11 Dig-Lig experiments | |||
- Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands. | |||
Latest revision as of 00:07, 27 September 2017
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Week 2 Day 4TSL - Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies. - Selected 2 white colonies from each plated and colony PCR performed. - Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct. - Decided to repeat the 11 Dig-Lig experiments - Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands.
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