IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/23: Difference between revisions

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==Floor Two=
==Floor Two==
The three overnight cultures were retrieved from the incubator. Cultures three and four were used to inoculate four fresh 10ml LB liquids that contained Kanamycin and Chlorophenicol antibiotics. 100μL from each culture inoculated a fresh LB, resulting in two cultures per original culture. They were left in the shaking incubator at 37°C until OD600 reached 0.4-0.6.
The plates and cultures from the previous day were retrieved. Colonies had grown on the plates, but neither them or the cultures had a red colour as aspected. 5ml from each culture was used to purify a soluble protein sample and the remaining 5ml from each was used for minipreps to retrieve the DNA. All samples were put into the -20°C freezer overnight. <br>
 
The sequencing reaction for 3a appeared to be unsuccessful.


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Revision as of 06:08, 12 September 2013

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Floor Two

The plates and cultures from the previous day were retrieved. Colonies had grown on the plates, but neither them or the cultures had a red colour as aspected. 5ml from each culture was used to purify a soluble protein sample and the remaining 5ml from each was used for minipreps to retrieve the DNA. All samples were put into the -20°C freezer overnight.
The sequencing reaction for 3a appeared to be unsuccessful.


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