IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/04: Difference between revisions
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Lucy Clark (talk | contribs) (Autocreate 2013/09/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Floor One== | ||
Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.<br> | |||
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent <i>E. coli</i> cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction. | |||
Revision as of 04:52, 25 September 2013
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Floor OnePerformed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.
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