IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03: Difference between revisions
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==Floor One== | ==Floor One== | ||
[[Image:GEL6.png|thumb| Fig 1. Gel image of the digested pMS82. Lane 1 and 2 are 1Kb+ ladders, and lanes 3 and 4 are the digested plasmid. Lane 5 is a control of undigested pMS82. The lower band in lanes 3 and 4 is the excised neomycin gene, but the band above that is unknown.]] | |||
Made 1% agarose gel.<br> | Made 1% agarose gel.<br> | ||
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br> | Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br> | ||
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products ''fig 1''.<br> | Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products ''fig 1'' found that there were two bands in addition to the undigested plasmid. Unsure what the middle band could be.<br> | ||
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | ||
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | ||
Revision as of 09:55, 29 September 2013
 iGEM Project name 1
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Floor One Made 1% agarose gel.
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