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 iGEM Project name 1
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Floor One
Made 1% agarose gel.
Filtered, concentrated and resuspended spore stocks in 20% glycerol.
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products fig 1.
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N.
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iGEM Project name 1
