IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/30: Difference between revisions
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The ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. <br> | The ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. <br> | ||
Minipreps were performed on the inoculated cultures of samples 3a and Bba_J04450 and left in the -20°C freezer. <br> | Minipreps were performed on the inoculated cultures of samples 3a and Bba_J04450 and left in the -20°C freezer. <br> | ||
The pellets of BL21 cells with | The pellets of BL21 cells with pET28AntA plasmid and BL21 cells with pET28AntA and AntGRFP plasmids were retrieved. The soluble and insoluble fractions were prepared and analysed on a 15% SDS-PAGE gel. Colonies one and two from the plate transformed with BL21 (pET28AntA + AntGRFP) cells looked unusual in the soluble and insoluble fractions. They appeared to contain unexpected DNA. But Colonies three and four from the same plate looked promising and would need further investigating to establish whether they contain two plasmids. | ||
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Revision as of 04:12, 11 September 2013
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Floor OnePerformed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse. Floor TwoThe ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. |