IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/15: Difference between revisions
Lucy Clark (talk | contribs) No edit summary |
(fix raw html notebook nav) |
||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Eleven</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Eleven</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
==Floor One== | |||
Made 4 x 500 ml and 2 x 1000 ml of SFM + Ny. | |||
Completed more lawns of single Streptomyces colonies. | |||
Decided to no longer re-streak contaminated plates as we have sufficient plates growing to produce a reasonable no. of spore stocks. | |||
==Floor Two== | ==Floor Two== | ||
The two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C. <br> | The two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C. <br> | ||
| Line 10: | Line 19: | ||
Two LB solutions were inoculated with BL21 PlySs and BL21 Rosetta PlySs seperately and incubated overnight at 37°C. | Two LB solutions were inoculated with BL21 PlySs and BL21 Rosetta PlySs seperately and incubated overnight at 37°C. | ||
[[Image:Bl21 Anta plates.jpg|thumb|Fig.1:Transformation of pETAntA plasmid into BL21 ''E.Coli''cells and spread onto LB agar + Kanamycin plates.]] | [[Image:Bl21 Anta plates.jpg|thumb|Fig.1:Transformation of pETAntA plasmid into BL21 ''E.Coli'' cells and spread onto LB agar + Kanamycin plates.]] | ||
Latest revision as of 23:11, 26 September 2017
 Week Eleven
|
 Main project page Previous entry Next entry
|
Floor OneMade 4 x 500 ml and 2 x 1000 ml of SFM + Ny. Completed more lawns of single Streptomyces colonies. Decided to no longer re-streak contaminated plates as we have sufficient plates growing to produce a reasonable no. of spore stocks.
Floor TwoThe two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C. 
| |
