IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/13: Difference between revisions
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Lucy Clark (talk | contribs) (Autocreate 2013/08/13 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Eleven</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Floor One== | ||
Isolated colonies from plated soil samples 61-76 that had characteristics of Streptomyces colonies. | |||
Re-streaked several contaminated plates. | |||
Filtered, concentrated and resuspended spore stocks (in 20 % glycerol). | |||
Started de-replicating plates - removing plates where it appeared we had duplicate single streaked colonies. | |||
Poured SFM + Apr/Hyg (30 ul per plate) for conjugation lawns - added Apr for colonies that received pAU3-45 and Hyg for those that received pMS82. Streaked lawns of conjugated Streptomyces colonies in preparation for spore stocks. | |||
==Floor Two== | |||
The plates that had been spread with the BL21 and BL21 star cells had no colonies. <br> | |||
DNA samples of our four biobricks were prepared and sent off for sequencing analysis. | |||
Revision as of 07:42, 22 August 2013
 Week Eleven
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Floor OneIsolated colonies from plated soil samples 61-76 that had characteristics of Streptomyces colonies. Re-streaked several contaminated plates. Filtered, concentrated and resuspended spore stocks (in 20 % glycerol). Started de-replicating plates - removing plates where it appeared we had duplicate single streaked colonies. Poured SFM + Apr/Hyg (30 ul per plate) for conjugation lawns - added Apr for colonies that received pAU3-45 and Hyg for those that received pMS82. Streaked lawns of conjugated Streptomyces colonies in preparation for spore stocks. Floor TwoThe plates that had been spread with the BL21 and BL21 star cells had no colonies.
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