IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/08: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Lucy Clark (talk | contribs) |
Lucy Clark (talk | contribs) |
||
| Line 7: | Line 7: | ||
==Floor Two== | ==Floor Two== | ||
A restriction enzyme digest was prepared using Nde1 and Xba1 to characterise our biobricks. The samples were analysed using agarose gel electrophoresis. The gel was stained with Etbr, this allowed us to define the smaller bands, but the marker become unclear ''fig 1''. | A restriction enzyme digest was prepared using Nde1 and Xba1 to characterise our biobricks. The samples were analysed using agarose gel electrophoresis. The gel was stained with Etbr, this allowed us to define the smaller bands, but the marker become unclear ''fig 1''. | ||
[[Image:08-08 etbr stain gel.JPG|thumb|Fig 1: Staining with Ethidium Bromide the gel from the analysis by restriction enzyme digest of the biobricks with Nde1 and Xba1 enzymes.]] | [[Image:08-08 etbr stain gel.JPG|thumb|Fig 1: Staining with Ethidium Bromide the gel from the analysis by restriction enzyme digest of the biobricks with Nde1 and Xba1 enzymes. Lanes 1-9 containing samples 3b1, J04450, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2, 2b1, 2b2, 2c1 respectively. ]] | ||
We began entering information about our biobricks into the registry | We began entering information about our biobricks into the registry | ||
Revision as of 04:04, 9 August 2013
 Week Ten
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Floor TwoA restriction enzyme digest was prepared using Nde1 and Xba1 to characterise our biobricks. The samples were analysed using agarose gel electrophoresis. The gel was stained with Etbr, this allowed us to define the smaller bands, but the marker become unclear fig 1.  We began entering information about our biobricks into the registry
| |
