IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/31: Difference between revisions
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==Floor Two== | ==Floor Two== | ||
A miniprep of the biobrick AntAgp | A miniprep of the biobrick AntAgp+Neo was prepared and analysed by gel electrophoresis. | ||
A colony from the E.coli BL21 plates was inoculated into LB with Kanamycin and Cholorophenicol antibiotics to perform protein over expression of the His tagged AntA σ factor. | A colony from the E.coli BL21 plates was inoculated into LB with Kanamycin and Cholorophenicol antibiotics to perform protein over expression of the His tagged AntA σ factor. | ||
[[Image:31-07.JPG|thumb|Analysis of biobrick AntAgp | [[Image:31-07.JPG|thumb|Analysis of biobrick AntAgp+Neo using Gel electrophoresis.]] | ||
==Outreach== | ==Outreach== | ||
Revision as of 03:47, 9 August 2013
 Week Nine
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Floor TwoA miniprep of the biobrick AntAgp+Neo was prepared and analysed by gel electrophoresis. A colony from the E.coli BL21 plates was inoculated into LB with Kanamycin and Cholorophenicol antibiotics to perform protein over expression of the His tagged AntA σ factor.  OutreachFinalizing posters for printing for the Forum event. Possibly, we will now have edible ants! Quality street idea as an analogy for decreasing anti-biotic discovery will be useful. Have been contacting local radio stations (Future Radio, and radio station Matt mentioned) to see about doing an interview + promotion of the Forum event. I have also contacted Lisa Horten from the UEA press office to see how we can publicise our project/forum event. Possible appearance in the EDP along with Matts lab.
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