IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/30

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Floor 2

Minipreps of grown cultures overnight were prepared, then samples were cut by (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. After incubation samples were analysed using agarose gel electrophoresis.

Restriction digest reactions using (i) EcoR1 and Pst1 (ii) Nde1 and Xba1. Lanes 1 and 7 are sample 1 colony 1, Lanes 2 and 8 are sample 1 colony 2. Lanes 3 and 9 are sample 2 colony 1, lanes 4 and 10 are sample 2 colony 2



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