IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29: Difference between revisions
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==Floor 2== | ==Floor 2== | ||
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. | |||
[[Image:29 july.JPG|thumb|Fig. ]] | [[Image:29 july.JPG|thumb|Fig. ]] | ||
Revision as of 21:12, 5 August 2013
 Week Nine
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Floor 2Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. 
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