IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/16: Difference between revisions
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(Autocreate 2013/07/16 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Seven</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Lab== | ||
===Floor Two=== | |||
More restriction digest reactions were performed, for all the samples as well as the original DNA as controls, using Pst1&Xba1 and Xba1&Nde1. After incubations samples were run on an agarose gel. | |||
In the meantime we prepared samples to send them for sequencing, by diluting the primers and checking the concentration of samples using the photometer. | |||
===Floor One=== | |||
Another 6 x 500 ml of SFM was made, autoclaved and poured into plates to set. | |||
Dilutions of 10<sup>-4</sup>, 10<sup>-6</sup>, 10<sup>-8</sup> and 10<sup>-10</sup> were plated on SFM + Ny and Cy for samples 12-38. | |||
All plates were incubated at 30°C for 7 days. | |||
==Outreach== | |||
Preliminary results from Lab on floor 1 suggest we may have to slightly change the Forum outreach activity angle. | |||
Revision as of 05:31, 25 September 2013
 Week Seven
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
LabFloor TwoMore restriction digest reactions were performed, for all the samples as well as the original DNA as controls, using Pst1&Xba1 and Xba1&Nde1. After incubations samples were run on an agarose gel. In the meantime we prepared samples to send them for sequencing, by diluting the primers and checking the concentration of samples using the photometer. Floor OneAnother 6 x 500 ml of SFM was made, autoclaved and poured into plates to set. Dilutions of 10-4, 10-6, 10-8 and 10-10 were plated on SFM + Ny and Cy for samples 12-38. All plates were incubated at 30°C for 7 days. OutreachPreliminary results from Lab on floor 1 suggest we may have to slightly change the Forum outreach activity angle.
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