IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/15: Difference between revisions
Lucy Clark (talk | contribs) (→Lab) |
|||
| (4 intermediate revisions by 3 users not shown) | |||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
==Lab== | ==Lab== | ||
===Floor Two=== | |||
Further digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1. | Further digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1. | ||
| Line 13: | Line 15: | ||
After incubation samples were run on a gel to analyse them. | After incubation samples were run on a gel to analyse them. | ||
===Floor One=== | |||
All soil samples received so far were numbered and decanted into uniform tubes (1-38). | |||
Samples 1-38 were diluted from 10<sup>-1</sup> up to 10<sup>-10</sup>. | |||
Soya flour mannitol (SFM) media was made in quantities of 6 x 500 ml, autoclaved for 1 hour then poured into plates to set. | |||
Dilutions of 10<sup>-2</sup>, 10<sup>-4</sup>, 10<sup>-6</sup>, 10<sup>-8</sup> and 10<sup>-10</sup> of samples 1-11 were plated onto SFM + 500 μL per 500 ml of Nystatin (Ny) and Cyclohexamide (Cy). | |||
Plates were incubated at 30°C for 7 days. | |||
==Outreach== | ==Outreach== | ||
Revision as of 05:30, 25 September 2013
 Week Seven
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
LabFloor TwoFurther digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1. (We were not supposed to use EcoR1 but there was no enough Xba1). After incubation samples were run on a gel to analyse them. Floor OneAll soil samples received so far were numbered and decanted into uniform tubes (1-38). Samples 1-38 were diluted from 10-1 up to 10-10. Soya flour mannitol (SFM) media was made in quantities of 6 x 500 ml, autoclaved for 1 hour then poured into plates to set. Dilutions of 10-2, 10-4, 10-6, 10-8 and 10-10 of samples 1-11 were plated onto SFM + 500 μL per 500 ml of Nystatin (Ny) and Cyclohexamide (Cy). Plates were incubated at 30°C for 7 days. OutreachCarried on e-mailing iGEm teams , but now focused on sediment after communications with Lab on floor 1. Ben Thompson arrangements. E-mailed iGEM contact (Nitwa ..) about the idea of an outreach registry, so that teams who are organizing a public engagement event (i.e. Forum) could have a infomrational resource pool to draw from, instead of starting from scrach each time. Will follow up iGEM (Westminster) as they have a simliar project and may be interested in such a resource and collaborating in its construction. Organized a meeting with Anne Osbourne (JIC) who spoke at this years SB6.0 SynBio conference. This is related to projects leading on from/inspired by iGEM and will focus on how iGEM bricks can be used in real research.
| |
